Integrative Physiology of Aging Lab
Department of Integrative Physiology
1725 Pleasant Street, 354 UCB
University of Colorado Boulder
Boulder, CO 80309
Office: 303.492.7365
E-Mail: [email protected]
Research Interests
My current research projects aim to understand how small molecule profiles in the blood may be related to, and predictive of, changes in physiological function with advancing age that are indicative of future disease risk. By using a new technique called "Metabolomics", we can measure thousands of circulating small molecules that provide a blueprint of systemic metabolism that may be representative of an individual's health state. Metabolomics is highly sensitive, detecting changes in circulating metabolites not revealed with traditional clinical blood panels. By pairing these molecular profiles with well-established clinical measures, we hope to better understand the aging process and uncover novel therapeutic targets to reduce the risk of chronic disease in aging populations. Overall, this work will help to identify individuals in need of clinical intervention prior to the onset of symptoms, improving treatment options and reducing healthcare costs.
Education
2014 - 2017 Ph.D. Integrative Physiology
University of Colorado Boulder
Advisor: Douglas Seals, Ph.D.
2012 - 2014 M.S. Integrative Physiology
University of Colorado Boulder
Advisor: Douglas Seals, Ph.D.
2006 - 2010 B.A. Integrative Physiology
University of Colorado Boulder
Experience
2014 - Present Graduate Research Assistant, Department of Integrative Physiology
University of Colorado Boulder, Boulder, CO
2014 - Present Research Affiliate, Department of Immunology and Microbiology
University of Colorado Anschutz Medical Campus, Aurora, CO
2013 - 2014 Research Affiliate, Integrated Department of Immunology
National Jewish Health, Denver, CO
2012 - 2014 Graduate Teaching Assistant, Department of Integrative Physiology
University of Colorado Boulder, Boulder, CO
2011 - 2012 Research Scientist, COR Innovations
Berkeley, CA
2010 - 2011 Research Assistant, Department of Integrative Physiology
University of Colorado Boulder, Boulder, CO
2008 - 2009 Sports Medicine Intern, Department of Athletics - Varsity Football
University of Colorado Boulder, Boulder, CO
Professional Developement
2014 National Jewish Health Metabolomics Workshop
2014 National Jewish Health, Denver, CO
2014 Mayo Clinic Metabolomics Workshop
2014 Mayo Clinic, Rochester, MN
Extramural Funding
Ruth L. Kirschstein Predoctoral Fellowship:
"Probing Molecular Mechanisms Underlying Functional Improvements to Interventions in Older Adults Using Metabolomics Analysis"
PI: Schwartz Agency: NIH National Institute on Aging
Type: T32 AG000279 Funding Period: 2014-2016
Honors/Awards
2013 Graduate School Young Scientist Travel Award, University of Colorado Boulder
2015 Early Career Scientist Research Excellence Award, National Institutes of Health
Professional Affiliations
2012-Present American Physiological Society
2012-Present American Heart Association
2015-Present Metabolomics Society
Invited Speaker Presentations
The Mayo Clinic Metabolomics Symposium: "Curcumin Supplementation Modifies the Plasma Metabolome and Improves Physiological Function in Middle-Aged and Older Adults", October 15th, 2016. The Mayo Clinic, Rochester, MN.
Publications
de Picciotto NE, Gano L*, Johnson LC*, Martens CR, Sindler AL, Mills KF, Imai S, Seals DR. Nicotinamide mononucleotide supplementation reverses vascular dysfunction and oxidative stress with aging in mice. Aging Cell. 11 mar 2016. DOI: 10.1111/acel.12461. (* denotes shared authorship).
Johnson LC, DeVan AE, Justice JN, Seals DR. Nitrate and Nitrite in Aging and Age-Related Disease. In, N. Bryan, J. Loscalzo (Eds.), Nitrate and Nitrite in Human Health and Disease, 2nd Edition, Springer International Publishing, 2016.
Justice JN*, Johnson LC*, DeVan AE, Cruickshank-Quinn C, Reisdorph N, Bassett CJ, Evans TJ, Brooks FA, Bryan NS, Chonchol MB, Giordano T, McQueen MB, Seals DB. Improved motor and cognitive performance with sodium nitrite supplementation is related to small metabolite signatures: a pilot trial in middle-aged and older adults. Aging (Albany NY) 7 (11), 1004.(* denotes shared first authorship)
DeVan AE, Johnson LC, Brooks FA, Evans TD, Justice JN, Cruickshank-Quinn C, Reisdorph N, Bryan NS, McQueen MB, Santos-Parker JR, Chonchol MB, Bassett CJ, Sindler AL, Giordano T, Seals DR. Effects of sodium nitrite supplementation on vascular function and related small metabolite signatures in middle-aged and older adults. J Appl Physiol (2015): jap-00879.
Justice JN, Gioscia-Ryan RA, Johnson LC, Battson ML, dePicciotto NE, Beck HJ, Jiang H, Sindler AL, Bryan NS, Enoka RM, Seals DR. Sodium nitrite supplementation improves motor function and skeletal muscle inflammatory profile in old male mice. J Appl Physiol (1985). 2015 Jan 15;118(2):163-9.
Sindler AL, Johnson LC, DePicciotto NE, Ahsan S, Weber S, Seals DR, Giosca-Ryan RA. Nitrite supplementation improves arterial mitochondrial health in old mice. FASEB J.
Johnson LC, de Picciotto NE, Cruickshank-Quinn C, Reisdorph N, Giordano T, DeVan AE, Seals DR, Sindler AL. Sodium nitrite supplementation alters the human metabolome in healthy middle-aged and older adults. FASEB J, 2014.
Battson ML, Gioscia-Ryan RA, Johnson LC, Seals DR, Justice JN. Sodium nitrite supplementation improves motor function and skeletal muscle inflammatory profile in old mice. FASEB J, 2014.
de Picciotto NE, Gano LB, Johnson LC, Eng JS, Weber S, Imai S, Seals DR, Sindler AL. Nicotinamide mononucleotide supplementation reverses vascular endothelial dysfunction and large elastic artery stiffness in old mice. FASEB J.
Johnson LC, Eng JS, Fleenor BS, Seals DR, Sindler AL. (2013). Circulating plasma factors contribute to age-associated arterial stiffness. FASEB J.27:lb666, 2013.
Eng JS, Pham BT, Johnson LC, Gioscia-Ryan RA, Seals DR, Fleenor BS. (2013). Voluntary aerobic exercise destiffens arteries of old mice via inhibition of NADPH Oxidase– and superoxide-dependent oxidative stress. FASEB J27:lb654, 2013.
My current research projects aim to understand how small molecule profiles in the blood may be related to, and predictive of, changes in physiological function with advancing age that are indicative of future disease risk. By using a new technique called "Metabolomics", we can measure thousands of circulating small molecules that provide a blueprint of systemic metabolism that may be representative of an individual's health state. Metabolomics is highly sensitive, detecting changes in circulating metabolites not revealed with traditional clinical blood panels. By pairing these molecular profiles with well-established clinical measures, we hope to better understand the aging process and uncover novel therapeutic targets to reduce the risk of chronic disease in aging populations. Overall, this work will help to identify individuals in need of clinical intervention prior to the onset of symptoms, improving treatment options and reducing healthcare costs.
Education
2014 - 2017 Ph.D. Integrative Physiology
University of Colorado Boulder
Advisor: Douglas Seals, Ph.D.
2012 - 2014 M.S. Integrative Physiology
University of Colorado Boulder
Advisor: Douglas Seals, Ph.D.
2006 - 2010 B.A. Integrative Physiology
University of Colorado Boulder
Experience
2014 - Present Graduate Research Assistant, Department of Integrative Physiology
University of Colorado Boulder, Boulder, CO
2014 - Present Research Affiliate, Department of Immunology and Microbiology
University of Colorado Anschutz Medical Campus, Aurora, CO
2013 - 2014 Research Affiliate, Integrated Department of Immunology
National Jewish Health, Denver, CO
2012 - 2014 Graduate Teaching Assistant, Department of Integrative Physiology
University of Colorado Boulder, Boulder, CO
2011 - 2012 Research Scientist, COR Innovations
Berkeley, CA
2010 - 2011 Research Assistant, Department of Integrative Physiology
University of Colorado Boulder, Boulder, CO
2008 - 2009 Sports Medicine Intern, Department of Athletics - Varsity Football
University of Colorado Boulder, Boulder, CO
Professional Developement
2014 National Jewish Health Metabolomics Workshop
2014 National Jewish Health, Denver, CO
2014 Mayo Clinic Metabolomics Workshop
2014 Mayo Clinic, Rochester, MN
Extramural Funding
Ruth L. Kirschstein Predoctoral Fellowship:
"Probing Molecular Mechanisms Underlying Functional Improvements to Interventions in Older Adults Using Metabolomics Analysis"
PI: Schwartz Agency: NIH National Institute on Aging
Type: T32 AG000279 Funding Period: 2014-2016
Honors/Awards
2013 Graduate School Young Scientist Travel Award, University of Colorado Boulder
2015 Early Career Scientist Research Excellence Award, National Institutes of Health
Professional Affiliations
2012-Present American Physiological Society
2012-Present American Heart Association
2015-Present Metabolomics Society
Invited Speaker Presentations
The Mayo Clinic Metabolomics Symposium: "Curcumin Supplementation Modifies the Plasma Metabolome and Improves Physiological Function in Middle-Aged and Older Adults", October 15th, 2016. The Mayo Clinic, Rochester, MN.
Publications
de Picciotto NE, Gano L*, Johnson LC*, Martens CR, Sindler AL, Mills KF, Imai S, Seals DR. Nicotinamide mononucleotide supplementation reverses vascular dysfunction and oxidative stress with aging in mice. Aging Cell. 11 mar 2016. DOI: 10.1111/acel.12461. (* denotes shared authorship).
Johnson LC, DeVan AE, Justice JN, Seals DR. Nitrate and Nitrite in Aging and Age-Related Disease. In, N. Bryan, J. Loscalzo (Eds.), Nitrate and Nitrite in Human Health and Disease, 2nd Edition, Springer International Publishing, 2016.
Justice JN*, Johnson LC*, DeVan AE, Cruickshank-Quinn C, Reisdorph N, Bassett CJ, Evans TJ, Brooks FA, Bryan NS, Chonchol MB, Giordano T, McQueen MB, Seals DB. Improved motor and cognitive performance with sodium nitrite supplementation is related to small metabolite signatures: a pilot trial in middle-aged and older adults. Aging (Albany NY) 7 (11), 1004.(* denotes shared first authorship)
DeVan AE, Johnson LC, Brooks FA, Evans TD, Justice JN, Cruickshank-Quinn C, Reisdorph N, Bryan NS, McQueen MB, Santos-Parker JR, Chonchol MB, Bassett CJ, Sindler AL, Giordano T, Seals DR. Effects of sodium nitrite supplementation on vascular function and related small metabolite signatures in middle-aged and older adults. J Appl Physiol (2015): jap-00879.
Justice JN, Gioscia-Ryan RA, Johnson LC, Battson ML, dePicciotto NE, Beck HJ, Jiang H, Sindler AL, Bryan NS, Enoka RM, Seals DR. Sodium nitrite supplementation improves motor function and skeletal muscle inflammatory profile in old male mice. J Appl Physiol (1985). 2015 Jan 15;118(2):163-9.
Sindler AL, Johnson LC, DePicciotto NE, Ahsan S, Weber S, Seals DR, Giosca-Ryan RA. Nitrite supplementation improves arterial mitochondrial health in old mice. FASEB J.
Johnson LC, de Picciotto NE, Cruickshank-Quinn C, Reisdorph N, Giordano T, DeVan AE, Seals DR, Sindler AL. Sodium nitrite supplementation alters the human metabolome in healthy middle-aged and older adults. FASEB J, 2014.
Battson ML, Gioscia-Ryan RA, Johnson LC, Seals DR, Justice JN. Sodium nitrite supplementation improves motor function and skeletal muscle inflammatory profile in old mice. FASEB J, 2014.
de Picciotto NE, Gano LB, Johnson LC, Eng JS, Weber S, Imai S, Seals DR, Sindler AL. Nicotinamide mononucleotide supplementation reverses vascular endothelial dysfunction and large elastic artery stiffness in old mice. FASEB J.
Johnson LC, Eng JS, Fleenor BS, Seals DR, Sindler AL. (2013). Circulating plasma factors contribute to age-associated arterial stiffness. FASEB J.27:lb666, 2013.
Eng JS, Pham BT, Johnson LC, Gioscia-Ryan RA, Seals DR, Fleenor BS. (2013). Voluntary aerobic exercise destiffens arteries of old mice via inhibition of NADPH Oxidase– and superoxide-dependent oxidative stress. FASEB J27:lb654, 2013.